Role of germinant receptors in Caco-2 cell-initiated germination of Bacillus cereus ATCC 14579 endospores.
نویسندگان
چکیده
Spores obtained from Bacillus cereus ATCC 14579 and mutant strains lacking each of seven germinant receptor operons were exposed to differentiated Caco-2 cells and monitored for germination. Spores of the gerI and gerL mutants showed a reduced germination response, pointing to a role for these receptors in Caco-2-induced germination.
منابع مشابه
gerR, a novel ger operon involved in L-alanine- and inosine-initiated germination of Bacillus cereus ATCC 14579.
Bacillus cereus endospores germinate in response to particular nutrients. Spores are able to sense these nutrients in the environment by receptors encoded by the gerA family of operons. Analysis of the Bacillus cereus ATCC 14579 genome revealed seven gerA family homologues. Using a transposon Tn917-based insertional mutagenesis approach followed by an enrichment procedure to select for l-alanin...
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We have tested a series of inosine analogs for their effect on germinating B. cereus 569 spores. Our results showed that although inosine (hypoxanthine nucleoside) causes spore germination by itself, the kinetic pathway exhibited complex and strongly cooperative character. Contrary to inosine’s germinating effect, the purine pathway degradation products xanthine, xanthosine, uric acid, hypoxant...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 75 4 شماره
صفحات -
تاریخ انتشار 2009